Reappraisal of Quantitative Gel Zymography for Matrix Metalloproteinases
نویسندگان
چکیده
منابع مشابه
Detection of Functional Matrix Metalloproteinases by Zymography
Matrix metalloproteinases (MMPs) are zinc-containing endopeptidases. They degrade proteins by cleavage of peptide bonds. More than twenty MMPs have been identified and are separated into six groups based on their structure and substrate specificity (collagenases, gelatinases, membrane type [MT-MMP], stromelysins, matrilysins, and others). MMPs play a critical role in cell invasion, cartilage de...
متن کاملEffects of blood collection methods on gelatin zymography of matrix metalloproteinases.
References 1. Tanumihardjo SA, Cheng JC, Permaesih D, Muherdiyantiningsih, Rustan E, Muhilal, et al. Refinement of the modified-relative-dose-response test as a method for assessing vitamin A status in a field setting: experience with Indonesian children. Am J Clin Nutr 1996;64: 966–71. 2. de Pee S, Yuniar Y, West CE, Muhilal. Evaluation of biochemical indicators of vitamin A status in breast-f...
متن کاملQuantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography.
We describe a sensitive zymography technique that utilizes an automated microfluidic platform to report enzyme molecular weight, amount, and activity (including k(cat) and K(m)) from dilute protein mixtures. Calf intestinal alkaline phosphatase (CIP) is examined in detail as a model enzyme system, and the method is also demonstrated for horseradish peroxidase (HRP). The 40 min assay has a detec...
متن کاملP 88: Matrix Metalloproteinases in Neuroinflammation
Matrix metalloproteinases (MMPs) are a family of neutral proteinases that are important in normal development, cellular differentiation or migration, angiogenesis, neurogenesis, wound repair, and a wide range of pathological processes such as oxidative stress and neuroinflammation. MMPs have been demonstrated to increase the permeability of the blood–brain barrier (BBB) by degrading the c...
متن کاملMatrix metalloproteinases
What makes an MMP an MMP? They all have a conserved zinc-binding site in their catalytic domain (HEXGHXXGXXH), and the sequence PRCGVPD in the propeptide domain. The cysteine residue serves to anchor the propeptide to the catalytic zinc, suppressing enzymatic activity in a manner that is not unlike a thumb in an open mouth. Release of this interaction results in proteolytic activity. MMPs are a...
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ژورنال
عنوان ژورنال: Journal of Clinical Laboratory Analysis
سال: 2014
ISSN: 0887-8013
DOI: 10.1002/jcla.21696